Basic Information |
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| Product Name： | 3,3',5,5'-TETRAMETHYLBENZIDINE |
| CAS： | 54827-17-7 |
| English Synonyms： | 3,3′,5,5′-TETRAMETHYLBENZIDINE (TMB) LIQUID SUBSTRATE SYSTEM FOR ELISA ; TMB, INSOLUBLE ; TETRAMETHYLBENZIDIN ; 3,3',5,5'-TETRAMETHYLBENZIDINE, TMB 99+% ; 3,3',5,5'-TETRAMETHYLBIPHENYL-4,4'-DIAMINE ; 3,3′,5,5′-TETRAMETHYLBENZIDINE ; TMB EASY ; 4,4'-BI-2,6-XYLIDINE ; BM BLUE ; 3,3',5,5'-TETRAMETHYLBENZIDINE, INSOLUBLE ; TMB REAGENT ; TETRAMETHYLBENZIDINE ; 3,3,5,5-TETRAMETHYLBENZIDINE ; 3,5,3',5'-TETRAMETHYLBENZIDINE ; TMB QUICK ; 3,3',5,5'-TETRAMETHYLBENZIDINE SUBSTRATE ; TMB+ ONE-STEP SUBSTRATE SYSTEM ; TMB BLUE ONE-STEP SUBSTRATE SYSTEM ; [1,1'-BIPHENYL]-4,4'-DIAMINE, 3,3',5,5'-TETRAMETHYL- ; TMB, SOLUBLE ; 3,3',5,5-TETRAMETHYLBENZIDINE ; TMB-M ; TMB SUBSTRATE ; 3,3',5,5'-TETRAMETHYLBENZIDINE, TMB ULTRA PURE 99.5+% ; 3,3',5,5'-TETRAMETHYL-[1,1'-BIPHENYL]-4,4'-DIAMINE ; TMB PLUS ; 3,3',5,5'-TETRAMETHYLBENZIDINE ; 3,3',5',5-TETRAMETHYLBENZIDINE ; TMB SOLUBLE REAGENT ; 4,4'-DIAMINO-3,3',5,5'-TETRAMETHYLBIPHENYL ; SURE BLUE TMB ; TMBZ ; 3355TMB ; TMB-B ; TMB-E ; 3,3',5,5'-TETRAMETHYLBENZIDINE LIQUID SUBSTRATE SYSTEM ; TMB BLOTTING PLUS ; 3,3',5,5'-TETRAMETHYL BENZIDINE ; TMB ; TMB LIQUID SUBSTRATE SYSTEM |
| MDL Number.： | MFCD00007748 |
| H bond acceptor： | 2 |
| H bond donor： | 2 |
| Smile： | Cc1cc(cc(c1N)C)c2cc(c(c(c2)C)N)C |
| InChi： | InChI=1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3 |
| InChiKey： | InChIKey=UAIUNKRWKOVEES-UHFFFAOYSA-N |
Property |
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| Melting Point： | 168-171 DEG C(LIT)/168-171 °C |
| Boiling Point： | DENSITY: 1 |
| Comments： | APPLICATION: 3,3',5,5'-TETRAMETHYLBENZIDINE (TMB) IS A NONCARCINOGENIC SUBSTITUTE (AMES TEST NEGATIVE) FOR BENZIDINE SUITABLE AS PEROXIDASE SUBSTRATE FOR USE IN ELISA PROCEDURES. THE SUBSTRATE PRODUCES A SOLUBLE END PRODUCT THAT IS PALE BLUE IN COLOR AND CAN BE READ SPECTROPHOTOMETRICALLY AT 370 OR 620-650 NM. THE TMB REACTION MAY BE STOPPED WITH 2 M H2SO4 (RESULTING IN A YELLOW COLOR), AND READ AT 450 NM. A SENSITIVE AND SPECIFIC REAGENT FOR THE DETECTION OF BLOOD, ASSAY OF HEMOGLOBIN, ASSAY OF PEROXIDASES RTECS: DV2300000 STORAGE TEMPERATURE: 2-8 DEG C UNSPSC: 12352100 WGK: 3 |
Safety information |
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| Symbol： |
GHS07
|
| Signal word： | Warning |
| Hazard statements： | H315-H319-H335 |
| Precautionary statements： | P261-P305 + P351 + P338 |
| hazard symbol： | Xi |
| Risk Code： | R:36/37/38 |
| Safe Code： | S:26-36 |
| WGK Germany： | 3 |
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