Basic Information |
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| Product Name： | 4',6-DIAMIDINO-2-PHENYLINDOLE DIHYDROCHLORIDE |
| CAS： | 28718-90-3 |
| English Synonyms： | 2-(4-AMIDINOPHENYL)INDOLE-6-CARBOXAMIDINE DIHYDROCHLORIDE ; 4',6-DIAMIDINO-2-PHENYLINDOLE, HYDROCHLORIDE ; 4',6-DIAMIDINE-2'-PHENYLINDOLE DIHYDROCHLORIDE ; DAPI ; DAPI DIHYDROCHLORIDE ; 4'',6-DIAMIDINO-2-PHENYLINDOLE, DIHYDROCHLORIDE ; 2-(4-AMIDINOPHENYL)-6-INDOLECARBAMIDINE DIHYDROCHLORIDE ; 4',6-DIAMIDINO-2-PHENYLINDOLE DIHYDROCHLORIDE ; 1H-INDOLE-6-CARBOXIMIDAMIDE, 2-[4-(AMINOIMINOMETHYL)PHENYL]-, DIHYDROCHLORIDE ; 4',6-DIAMIDINO-2-PHENYLINDOLE, 2HCL ; 4',6-DIAMIDINE-2-PHENYLINDOLE DIHYDROCHLORIDE ; 4',6'-DIAMIDINO-2-PHENYLINDOLE DIHYDROCHLORIDE |
| MDL Number.： | MFCD00012681 |
| H bond acceptor： | 5 |
| H bond donor： | 5 |
| Smile： | c1cc(ccc1c2cc3ccc(cc3[nH]2)C(=N)N)C(=N)N.Cl.Cl |
| InChi： | InChI=1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H |
| InChiKey： | InChIKey=FPNZBYLXNYPRLR-UHFFFAOYSA-N |
Property |
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| Boiling Point： | MP: APPROX 330 DEG C (DECOMPOSITION) |
| Comments： | APPLICATION: DAPI IS SEVERAL TIMES MORE SENSITIVE THAN ETHIDIUM BROMIDE FOR STAINING DNA IN AGAROSE GELS. IT MAY BE USED FOR PHOTOFOOTPRINTING OF DNA, TO DETECT ANNEALED PROBES IN BLOTTING APPLICATIONS BY SPECIFICALLY VISUALIZING THE DOUBLE-STRANDED COMPLEX, AND TO STUDY THE CHANGES IN DNA AND ANALYZE DNA CONTENT DURING APOPTOSIS USING FLOW CYTOMETRY. DAPI STAINING HAS ALSO BEEN SHOWN TO BE A SENSITIVE AND SPECIFIC DETECTION METHOD FOR MYCOPLASMA APPLICATION: FORMS HIGHLY FLUORESCENT COMPLEXES WITH DNA FROM VARIOUS SOURCES BIOCHEM/PHYSIOL ACTIONS: CELL PERMEABLE FLUORESCENT MINOR GROOVE-BINDING PROBE FOR DNA. BINDS TO THE MINOR GROOVE OF DOUBLE-STRANDED DNA (PREFERENTIALLY TO AT RICH DNA), FORMING A STABLE COMPLEX WHICH FLUORESCES APPROXIMATELY 20 TIMES GREATER THAN DAPI ALONE FLUORESCENCE: LAMBDAEM 454 NM (DAPI-DNA-KOMPLEX) FLUORESCENCE: LAMBDAEM 488 NM (NUR DAPI) FLUORESCENCE: LAMBDAEX 340 NM FLUORESCENCE: LAMBDAEX 364 NM WGK: 3 |
Safety information |
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| hazard symbol： | Xi |
| Risk Code： | R:36/37/38 |
| Safe Code： | S:26,36 |
| WGK Germany： | 3 |
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