IMMOBILIZED E COLI LYSATE_Chemical Cloud Database

IMMOBILIZED E COLI LYSATE

Basic Information
Product Name：	IMMOBILIZED E COLI LYSATE
English Synonyms：	IMMOBILIZED E COLI LYSATE
MDL Number.：	MFCD00163995
H bond acceptor：	0
H bond donor：	0
InChi：	InChI=1S//
InChiKey：	InChIKey=MOSFIJXAXDLOML-UHFFFAOYSA-N
Property
Comments：	COMMENT 1: HIGH BACKGROUND, OR LOW SIGNAL:NOISE RATIO, IS OFTEN A PROBLEM WHEN SCREENING LIBRARIES COMMENT 2: CRUDE ANTISERA AND ASCITES FLUID OFTEN CONTAIN IGG COMPONENTS THAT BIND TO ESCHERICHIA COLI PROTEINS COMMENT 3: THIS COULD BE ESPECIALLY PROBLEMATIC IF THE TITER OR BINDING AFFINITY OF THE E COLI BINDING ANTIBODIES IS HIGHER THAN THAT OF THE ANTIBODY TO THE PROTEIN OF INTEREST, MAKING THE BACKGROUND OF THE FALSE POSITIVE PLAQUES HIGH COMMENT 4: TO MAKE REMOVAL OF E COLI ANTIBODIES CLEANER AND EASIER, PIERCE HAS IMMOBILIZED E COLI PROTEINS ON A SOLID SUPPORT COMMENT 5: WHEN A SAMPLE IS PASSED OVER THE COLUMN, THE PURIFIED ANTIBODY IS COLLECTED IN THE VOID VOLUME COMMENT 6: THE CONTAMINATING E COLI ANTIBODIES ARE ABSORBED ONTO THE MATRIX, AND THE PURIFIED ANTISERA IS COLLECTED ECONOMICALLY PRICED IN JUMBO QUANTITIES FOR CLEAN, EASY REMOVAL OF E COLI-REACTIVE ANTIBODIES
Information：	GEL OF E COLI PROTEINS FROM STRAIN BMH 71-18 IMMOBILIZED ON 4% CROSS-LINKED BEADED AGAROSE
Safety information

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